发明名称 DETECTION OF BRAF V600E MUTATION BY ALLELE SPECIFIC REAL TIME QUANTITATIVE PCR (AS-QPCR) USING LOCKED NUCLEIC ACIDS PRIMERS AND BEACON PROBES
摘要 Method for conducting an allele specific polymerase chain reaction which comprises subjecting DNA which may contain either or both of first and second alleles to a polymerase chain reaction utilizing a primer pair in which one of the primers is complementary to said first allele, but which primer forms a mismatch with said second allele at the 3'-terminal nucleotide of the primer, and utilizing a DNA polymerase wherein said first allele is specifically amplified but no amplification of said second allele occurs.
申请公布号 WO2011104694(A3) 申请公布日期 2012.12.13
申请号 WO2011IB50814 申请日期 2011.02.25
申请人 GAMMAGENETICS SARL;MORANDI, LUCA 发明人 MORANDI, LUCA
分类号 C12Q1/68 主分类号 C12Q1/68
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