发明名称 |
HOST, TRANSFORMANT, METHOD FOR PRODUCING THE TRANSFORMANT, AND METHOD FOR PRODUCING HETEROGENEOUS PROTEIN CONTAINING O-GLYCOSIDE TYPE SUGAR CHAIN |
摘要 |
The object is to provide a transformant which can produce a heterologous protein having a structurally controlled O-linked sugar chain having an O-Man-Gal disaccharide structure, a method for producing the transformant by using Schizosaccharomyces pombe as the host, and provide a host for producing the transformant and a method for producing an O-glycosylated heterologous protein. An Schizosaccharomyces pombe host having no omh1 gene or an inactivated omh1 gene in its chromosomes for producing an O-glycosylated heterologous protein having an O-linked sugar chain having an O-Man-Gal disaccharide structure by expression of the heterologous protein by a genetic engineering technique and subsequent glycosylation of the expressed heterologous protein. A transformant from the host, a method for producing the transformant and a method for producing an O-glycosylated heterologous protein by using the transformant. |
申请公布号 |
EP2341139(A4) |
申请公布日期 |
2012.12.12 |
申请号 |
EP20090817834 |
申请日期 |
2009.09.30 |
申请人 |
ASAHI GLASS COMPANY, LIMITED |
发明人 |
TAKEGAWA, KAORU;HAMA, YUKO;TOHDA, HIDEKI |
分类号 |
C12N9/10;C12N1/19;C12P21/00 |
主分类号 |
C12N9/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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