发明名称 |
Method of enhancing recombinant protein production |
摘要 |
The present invention provides a method of enhanced protein production that comprises the step of expressing a recombinant gene encoding the protein in eukaryotic cells under conditions in which cleavage of the pro-domain of the protein is inhibited or eliminated. Generally the method of the present invention includes the step of inhibiting or altering the cleavage of a pro-domain of a recombinant protein of interest in order to increase the amount of recombinant protein secreted from a eukaryotic cell. Recombinant proteins that can be prepared using the method of this invention include members of the transforming growth factor-&bgr; (TGF-&bgr;) superfamily, such as bone morphogenetic proteins. Also provided are genetically engineered cells and polynucleotides for performing the method of the invention. |
申请公布号 |
US8329429(B2) |
申请公布日期 |
2012.12.11 |
申请号 |
US20090626441 |
申请日期 |
2009.11.25 |
申请人 |
PEEL SEAN A. F.;CLOKIE CAMERON M. L.;TURNER JEFFREY D.;INDUCE BIOLOGICS INC. |
发明人 |
PEEL SEAN A. F.;CLOKIE CAMERON M. L.;TURNER JEFFREY D. |
分类号 |
C12N15/02;C07K14/51;C12N5/07 |
主分类号 |
C12N15/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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