| 摘要 |
<p>PURPOSE: A DNA aptamer which specifically binds on the surface of probiotics of Listeria monocytogenes is provided to detect the probiotics in a sample of processed food products, dairy products, water, cookers, and water resources. CONSTITUTION: A DNA aptamer specifically binds on the surface of probiotics of Listeria monocytogenes. The DNA aptamer is an oligonucleotide with 90% or more homology with a base selected from sequence numbers 1-12. A composition for detecting probiotics of Listeria monocytogenes contains the DNA aptamer as an active ingredient. A kit for detecting the probiotics of Listeria monocytogenes contains the DNA aptamer as an active ingredient. A method for detecting the probiotics comprises: a step of contacting the DNA aptamer with a sample which is considered to possibly have the probiotics; and a step of identifying the probiotics conjugated with the DNA aptamer. [Reference numerals] (AA) Preparing ssDNA aptamer pool; (BB) Forming ssDNA aptamer structure; (CC) Culturing Listeria monocytogenes; (DD) Washing the Listeria monocytogenes; (EE) Inducing conjugation between Listeria monocytogenes and ssDNA; (FF) Collecting ssDNA conjugated with Listeria monocytogenes; (GG) Cloning into T-vector for sequence acquiring; (HH) In case of processing following round</p> |
