发明名称 |
Swellable (meth)acrylate surfaces for culturing cells in chemically defined media |
摘要 |
Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.
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申请公布号 |
US8329469(B2) |
申请公布日期 |
2012.12.11 |
申请号 |
US20090362974 |
申请日期 |
2009.01.30 |
申请人 |
FADEEV ANDREI G.;GEHMAN JENNIFER;MARTIN ARTHUR WINSTON;MELKOUMIAN ZARA;SHOGBON CHRISTOPHER B.;WEBER DAVID MICHAEL;ZHOU YUE;GERON CORPORATION |
发明人 |
FADEEV ANDREI G.;GEHMAN JENNIFER;MARTIN ARTHUR WINSTON;MELKOUMIAN ZARA;SHOGBON CHRISTOPHER B.;WEBER DAVID MICHAEL;ZHOU YUE |
分类号 |
C12N5/00;C12N11/00 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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地址 |
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