发明名称 |
Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo |
摘要 |
Methods are provided for labeling cellular glycans bearing azide groups via fluorescent labeling comprising Cu(I)-catalyzed [3+2] cycloaddition of a probe comprising alkynyl group. Generation of fluorescent probes from a nonfluorescent precursor, 4-ethynyl-N-ethyl-1,8-naphthalimide, by Cu(I)-catalyzed [3+2] cycloaddition of the alkyne group of the probe to an azido-modified sugar are provided. Incorporation of azido-containing fucose analog into glycoconjugates via the fucose salvage pathway are disclosed. Fluorescent visualization of fucosylated cells by flow cytometry of cells treated with 6-azidofucose labeled with click-activated fluorogenic probe or biotinylated alkyne is disclosed. Visualization of intracellular location of fucosylated glycoconjugates by fluorescence microscopy are disclosed. |
申请公布号 |
US8329413(B2) |
申请公布日期 |
2012.12.11 |
申请号 |
US201113053192 |
申请日期 |
2011.03.21 |
申请人 |
WONG CHI-HUEY;HSU TSUI-LING;HANSON SARAH R;SAWA MASAAKI;ACADEMIA SINICA |
发明人 |
WONG CHI-HUEY;HSU TSUI-LING;HANSON SARAH R;SAWA MASAAKI |
分类号 |
G01N33/53 |
主分类号 |
G01N33/53 |
代理机构 |
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