发明名称 |
METHOD FOR DETECTION OF BOVINE BETA-LACTOGLOBULINE VERSION B* |
摘要 |
FIELD: medicine.SUBSTANCE: method provides amplification of a beta lactoglobuline gene fragment of the length of 241 base pairs (positions 1821-2061 to Z48305) which contains the mutation C?A in position 2036 to Z48305 relevant to upstream position 215 of a translation initiation site of the beta lactoglobuline gene. The fragment is modified by the introduction of a restriction site; the following analysis of reaction products is assisted by RFLP-analysis with the use of Mspl restriction nuclease. The absence of the restriction site corresponds to allele B*.EFFECT: method enables effective and reliable diagnostics at all the stages of animal's ontogenesis that in turn makes it possible to use it in selection practice as a marker for production of low beta-lactoglobuline milk at all the stages of animal's ontogenesis.2 dwg, 1 ex |
申请公布号 |
RU2469088(C1) |
申请公布日期 |
2012.12.10 |
申请号 |
RU20110119894 |
申请日期 |
2011.05.18 |
申请人 |
OBSHCHESTVO S OGRANICHENNOJ OTVETSTVENNOST'JU "BIOSTRIM" |
发明人 |
GORELOV PETR VALER'EVICH;ZINOV'EVA NATALIJA ANATOL'EVNA;GLADYR' ELENA ALEKSANDROVNA;CHINAROV JURIJ IVANOVICH |
分类号 |
C12N15/00 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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