摘要 |
Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber. |
申请人 |
THE UNIVERSITY OF BRITISH COLUMBIA |
发明人 |
HANSEN, CARL LARS GENGHIS;VANINSBERGHE, MICHAEL;WHITE, ADAM;PETRIV, OLEH;LEAVER, TIM;SINGHAL, ANUPAM;BOWDEN, WILLIAM;LECAULT, VERONIQUE;DA COSTA, DAN;WU, LEO;RUSSELL, GEORGIA;SIKORSKI, DAREK |