GENE TARGETING VECTOR, METHOD FOR MANUFACTURING SAME, AND METHOD FOR USING SAME

摘要

Provided is a gene targeting vector capable of highly efficient gene targeting. The gene targeting vector characterized in that a DNA sequence capable of bicistronic expression is present 5' upstream of a selection marker. A method for producing the gene targeting vector, including linking a DNA fragment that is homologous to a 5' upstream region of the target site, a selection marker where a DNA sequence capable of bicistronic expression is present 5' upstream, and a DNA fragment that is homologous to a 3' downstream region of the target site.