REAGENTS AND METHODS FOR PRRSV DETECTION

摘要

The present application relates to the detecting reagents and detection methods of porcine reproductive and respiratory virus. In one aspect, the present disclosure provides immunogenic fragments that encoding non-structural protein 2 (Nsp2). Particularly, the immunogenic fragments as provided herein are absent in an Nsp2 protein of an attenuated PRRSV but are present in an Nsp2 protein of a wild type PRRSV. The present disclosure also provides oligonucleotide primers that are useful in distinguishing an attenuated PRRSV from a wild type PRRSV. The present application further provides detection methods and detecting reagents for PRRSV, and also methods of distinguishing pigs immunized by an attenuated PRRSV from pigs infected with a wild type PRRSV.