METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELLS USING A DEDIFFERENTIATION ENHANCER

摘要

The present invention relates to a method for preparing induced pluripotent stem cells (iPS cells), comprising: (a) culturing human-derived somatic cells, into which genes encoding differentiation-inducing factors are introduced, in a medium containing one or more dedifferentiation enhancers selected from a group consisting of a protein kinase C inhibitor, a histone deacetylase inhibitor, and a bone morphogenetic protein (BMP) pathway blocker; and (b) separating embryonic stem cell-like colonies from the culture obtained in step (a). The method of the present invention may enable the preparation of induced pluripotent stem cells with high efficiency, as well as the preparation of induced pluripotent stem cells under xenopathogen-free and feeder cell-free conditions.