| 摘要 |
Engineered strains of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 50 weight percent of eicosapentaenoic acid [EPA], anω-3 polyunsaturated fatty acid, in the total oil fraction are described. These strains over-express heterologousΔ9 elongases,Δ8 desaturases,Δ5 desaturases,Δ17 desaturases,Δ12 desaturases and C16/18 elongases, and optionally over-express diacylglycerol cholinephosphotransferases. Preferred gene knockouts are described, as are methods for producing EPA within the host cells and products comprising EPA from the optimized Yarrowia lipolytica strains.
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