发明名称 |
Process for high-throughput DNA methylation analysis |
摘要 |
There is disclosed an improved high-throughput and quantitative process for determining methylation patterns in genomic DNA samples based on amplifying modified nucleic acid, and detecting methylated nucleic acid based on amplification-dependent displacement of specifically annealed hybridization probes. Specifically, the inventive process provides for treating genomic DNA samples with sodium bisulfite to create methylation-dependent sequence differences, followed by detection with fluorescence-based quantitative PCR techniques. The process is particularly well suited for the rapid analysis of a large number of nucleic acid samples, such as those from collections of tumor tissues. |
申请公布号 |
US8323890(B2) |
申请公布日期 |
2012.12.04 |
申请号 |
US20090476981 |
申请日期 |
2009.06.02 |
申请人 |
LAIRD PETER W.;CARROLL CINDY A.;DANENBERG KATHLEEN D.;THE UNIVERSITY OF SOUTHERN CALIFORNIA |
发明人 |
LAIRD PETER W.;CARROLL CINDY A.;DANENBERG KATHLEEN D. |
分类号 |
C07H21/04;G01N33/53;C12N15/09;C12Q1/68;G01N21/78;G01N33/566;G01N33/58 |
主分类号 |
C07H21/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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