Liquid chromatography-mass spectrometer and its uses for the selective identification of immunogenic epitopes

摘要

A liquid chromatography-mass spectrometer device for analysing a sample comprising a pump arrangement, an analytical column, an electro spray ionization unit and a mass spectrometer, wherein the pump arrangement is constructed and arranged for providing a nanoscale flow to the analytical column, wherein the analytical column comprises a stationary phase for liquid chromatography and wherein the analytical column has an interior diameter of less than 200 microns, wherein the electro spray ionization unit comprises an emitter for electro spraying positioned downstream of the analytical column in the flow path of the sample, said emitter having an interior diameter of less than 70 microns, wherein the mass spectrometer is positioned downstream of the emitter, the liquid chromatography-mass spectrometer device comprising at least two-dimensional chromatography, wherein the first dimension comprises at least strong cation exchange (SCX) and the second dimension comprises reversed phase chromatography, wherein an elution solvent is a salt-free solution for both the first and second dimension of the two-dimensional chromatography.