摘要 |
<p>An application of luminol directly bonded gold nanoparticles in immunoassay is provided. An immunoassay probe with luminol directly bonded gold nanoparticles is composed of antibody labeled with luminol directly bonded gold nanoparticles, wherein the luminol directly bonded gold nanoparticles are prepared by a one-pot method through the reduction of chlorauric acid by luminol. A chemiluminescence immunoassay based on the luminol directly bonded gold nanoparticles immunoassay probe and a kit for performing the assay are also provided. The chemiluminescence immunoassay can be used for determining various analytes in samples. The probe and the assay can be applied to clinical diagnosis and treatment, pharmaceutical analysis, food safety assessment and environmental monitoring.</p> |