Vaccine composition comprising attenuated and immunopotentiated mutant of Salmonella Enteritidis for preventing salmonellosis

摘要

PURPOSE: A living bacteria vaccine composition for salmonellosis prevention including attenuating,mutant strain of immunopotentiation salmonella enteritidis is provided to enhance defense mechanism. CONSTITUTION: A living bacteria vaccine composition for salmonellosis prevention includes attenuating mutant strain of salmonella enteritidis, an immunopotentiation mutant strain which expresses LTB (heat-labile toxin subunit B) of Escherichia coli or a combination thereof. The combination of the immunopotentiation mutant strain and attenuating mutant strain is mixed at the ratio of bacteria numbers of 1:2-1:10. The salmonellosis is domestic fowls salmonellosis or human body salmonellosis. The domestic fowls salmonellosis is avian paratyphoid infection. The avian is hen, duck, turkey, quail, waterfowl, pigeon, and canary. The human body salmonellosis is sitotoxism. [Reference numerals] (AA) Salmonella EnteritidisΔlonΔcpxR mutant; (BB) Conservation, culturing; (CC) Vaccinating layer in various conditions; (DD) Verifying defensive power; (EE) Layer vaccination program for defending salmonella infection; (FF) Manufacturing; (GG) Side effect testing; (HH) Excellent safety confirming; (II) Defensive immunity testing; (JJ) Excellent immune confirming; (KK) Outdoor strain inoculating; (LL) Defensive power checking; (MM) Excellent defensive power checking; (NN) After vaccinating; (OO) Clinical symptoms; (PP) Egg contamination; (QQ) Environmental contamination; (RR) Cell-mediated immunity(lymphocyte proliferation reaction, FACS); (SS) Mucous membrane immunity(secretory type IgA); (TT) Humoral immunity (plasma IgG); (UU) After the outdoor strain is infected by dogen; (VV) Inner organ lesion; (WW) Infection checking(virus isolation); (XX) Spawning rate checking; (YY) Egg contamination checking(virus isolation)