发明名称 |
Solid phases optimized for chemiluminescent detection |
摘要 |
<p>Solid supports for chemiluminescent assays are provided. The solid support includes a plurality of probes covalently or physically attached to the support surface and a chemiluminescent enhancing moiety incorporated onto the surface or into the bulk of the support. The solid support can be a multi-layered support including an upper probe binding layer (e.g., an azlactone polymer layer or porous functional polyamide layer) adjacent to a cationic microgel layer. The azlactone-functional polymer can be a copolymer of dimethylacrylamide and vinylazlactone crosslinked with ethylenediamine. The cationic microgel layer can be a cross-linked quaternary onium salt containing polymer. A method and a kit for conducting chemiluminescent assays using the solid supports is also provided. The kit comprises a dioxetane substrate, a biopolymer probe-enzyme complex, and a solid support. The solid support can be an azlactone functional polymer layer adjacent to a acationic microgel layer; a porous polyamide functional layer adjacent to a cationic microgel layer; or a quaternized azlactone functional polymer layer.</p> |
申请公布号 |
EP2163897(B1) |
申请公布日期 |
2012.11.28 |
申请号 |
EP20090016129 |
申请日期 |
2003.01.17 |
申请人 |
APPLIED BIOSYSTEMS, LLC |
发明人 |
EDWARDS, BROOKS;GEISER, TIMOTHY;MENCHEN, STEVEN, M.;VOYTA, JOHN |
分类号 |
G01N33/532;G01N33/545;C12N15/09;C40B40/06;C40B40/10;G01N21/78;G01N33/533;G01N33/543;G01N33/547;G01N33/548 |
主分类号 |
G01N33/532 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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