| 摘要 |
<p>The invention generally relates to methods for assuring amplification of an abnormal nucleic acid that is present as a percentage of total nucleic acid in a sample. In certain embodiments, methods of the invention involve providing a sample from a subject, in which the sample includes a total of nucleic acids, in which a percentage of the total are abnormal nucleic acids, extracting the total of nucleic acids from the sample, quantitatively analyzing the extracted nucleic acids, thereby determining an amount of amplifiable nucleic acids in the sample, and providing an amount of the nucleic acids for an amplification reaction that assures amplification of the abnormal nucleic acids in the sample, in which the provided amount is based on results from the quantitatively analyzing step.</p> |
