METHOD OF IMPROVING T CELL RECEPTORS

摘要

A method of increasing the affinity and/or decreasing the off-rate of a given TCR specific for a given target pMHC, comprising creating a plurality of TCRs having an .alpha. chain CDR2 sequence and/or a .beta. chain CDR2 sequence different from the corresponding CDR2 sequence(s) of the given TCR but having the same .alpha. and .beta. CDR1 and CDR3 sequences as the given TCR, determining the affinity and/or off-rate of members of said plurality of TCRs for the target pMHC, and selecting one or more members having at least a 10-fold greater affinity for the target pMHC than the given TCR and/or a 10-fold slower off-rate for the target pMHC than the given TCR.