| 摘要 |
FIELD: chemistry.SUBSTANCE: group of inventions relates to biotechnology, particularly for culturing cells/tissue, as well as plant cells. The disposable apparatus for culturing and collecting plant tissue and/or cells has a flexible container with volume of not less than 400 l, having gas-exchange channels and a collection channel. The apparatus can be used continuously for at least two successive culturing/collection cycles with values or range of values of parameters selected from at least one of the following values or ranges of values: height to volume ratio from about 0.06 to 1 cm/l; gas pressure at the input from about 1 bar to 5 bar; input gas density per cross-sectional area from about 20 inputs per square metre to 70 inputs per square metre; rate of aeration at the input from about 0.05 to 0.12 gas volume per medium volume per minute; gas bubble volume at the input from about 20 cubic millimetre to 1800 cubic millimetre. The method and system for culturing and collecting plant tissue and/or plant cells employ the disposable apparatus.EFFECT: invention provides high output of the end product while reducing cost.21 cl, 7 tbl, 20 dwg |
