METHOD OF DETECTING ANTIBODIES TO MYCOBACTERIUM LEPRAE ON SOLID CARRIER

摘要

FIELD: medicine.SUBSTANCE: claimed is method of detecting antibodies to Mycobacterium leprae (M.leprae) on solid carrier. Places of application of components of immunologic reaction in ELISA on solid carrier (fluoroplastic tape) are sensitised with antigen from ultrasound disintegrate (sonicate) of M.leprae in dose 5 mcg/ml in volume 20 mcl for each sample of patients' blood serum. After that immunoperoxidase conjugate against human IgG is applied in the same volume on the places of previous location of reaction components. Unbound antigen from sensitised tape is removed with buffer solution (BPST) after each stage of reaction. Reaction results are estimated visually by difference in substrate mixture colouring.EFFECT: simplification of method of detection of antibodies to Mleprae due to application in ELISA of solid carrier-fluoroplastic tape.2 ex