DNA extraction method for bursaphelenchus xylophilus from wood chips, lamp primer set for bursaphelenchus xylophilus, and detection method for bursaphelenchus xylophilus from wood chips

摘要

To extract and detect Bursaphelenchus xylophilus DNA without isolating Bursaphelenchus xylophilus from a piece of wood taken from a tree belonging to the genus Pinus and the like, provided are a method for extracting Bursaphelenchus xylophilus DNA from a collected piece of wood that includes Bursaphelenchus xylophilus, a LAMP primer set including primers that anneal to a specific region of Bursaphelenchus xylophilus DNA, and a method for detecting Bursaphelenchus xylophilus from a piece of wood by amplifying a DNA by a LAMP method using this primer set.