METHOD FOR EXPRESSING A TARGET PROTEIN AT THE SURFACE OF A VACUOLE, AND DRUG DELIVERY CARRIER

摘要

The present invention relates to a method for expressing a target protein at the surface of a vacuole of a microorganism, and to a drug delivery carrier. More particularly, the present invention relates to a method comprising the steps of: (a) creating a vector including a nucleotide sequence encoding a fusion protein containing a target protein and a vacuole-targeting transmembrane protein; (b) transforming a microorganism containing a vacuole using said vector; and (c) expressing the fusion protein from the transformed microorganism. According to the present invention, the expressed fusion protein targets the vacuole and the target protein of the fusion protein is expressed at the surface of the vacuole. The present invention relates to a method for preparing a drug delivery carrier which is safe and easily applied in a clinical setting by fusion expressing the target protein and vacuole targeting transmembrane protein on the surface of a vacuole of a yeast. Further, the present invention relates to a method for preparing a drug delivery carrier which is cost-efficient when the method of the present invention is industrialized, using yeast having more economically advantageous culturing conditions.