摘要 |
The invention relates to organisms and compositions comprising one or more stem cells or one or more embryos, wherein the one or more stem cells or one or more embryos comprise one or more of the following mutations: (i) a deletion mutation; (ii) a knockout mutation; and/or (iii) an addition of a heterologous nucleic acid sequence; wherein the one or more mutations of (i), (ii), and/or (iii) are site-specific mutations caused by a Xanthomonas TAL nuclease (XTN). The invention also relates to method of mutating an embryo, iPS cell, stem cell, or more particularly a spermatogonial stem cell by exposing the nucleic acid sequence contained within such embryos or cell with a Xanthomonas TAL nuclease. |
申请人 |
TRANSPOSAGEN BIOPHARMACEUTICALS, INC.;THE GENERAL HOSPITAL CORPORATION;OSTERTAG, ERIC, M.;CRAWFORD, JOHN, S.;JOUNG, J., KEITH |
发明人 |
OSTERTAG, ERIC, M.;CRAWFORD, JOHN, S.;JOUNG, J., KEITH |