| 摘要 |
Methods and an apparatus according to the present invention, can be used in capture/enrichment, gene expression profiling and targeted sequencing. Provided are methods for improving accuracy and stringency of microarrays and/or other genomic analysis methods relying on nucleic acid hybridization and melting curve analysis by controlling surface chemistry. This method comprises producing a positively charged surface or surface coating, on the surface of microarray slides or other types of surfaces similarly purposed, such as micro beads, which enhances melting curve analysis to the point of allowing detection or differentiation of small changes in sequences between nucleic acid binding partners. Also provided is an improved microarray reader machine, to collect melting curve data on microarray slides. The accuracy or resolution of melting curve analysis was to be sufficient to distinguish between the melting of perfect matched dsDNA and dsDNA with the smallest possible change in sequence, a one base pair mismatch. |
