GENETICALLY MODIFIED PIG AS A CANCER PRONE MODEL

摘要

The present invention relates to a genetically modified pig and porcine cells comprising a genetically modified porcine p53 sequence. Methods to produce such a genetically modified pig are herein provided.In particular, the pig and cells are generated using zinc finger nuclease-mediated or Transcription activator-like effector nuclease-mediated technologies. Nucleases targeting porcine p53 nucleic acid sequences as well as nucleic acid sequences encoding said nucleases are herein described. Also herein provided are methods of assessing effects of agents in animals and cells comprising a genetically modified porcine p53 sequence. The pig according to the present invention can advantageously be used as a proliferative, preferably cancer,prone model. Pig and cells herein described can be used in toxicology and in oncology researches in order to identify new biomarkers of cancer and/or new compounds for preventing, treating or alleviating proliferative diseases such as cancer.