发明名称 |
PROBE BASED NUCLEIC ACID DETECTION |
摘要 |
The invention provides a method for detecting a target nucleotide sequence by tagging the nucleotide sequence with a nucleotide tag, providing a probe oligonucleotide with a melting temperature Tm1, comprising a regulatory sequence and a nucleotide tag recognition sequence; incorporating the probe oligonucleotide into the tagged polynucleotide in a polynucleotide amplification reaction, providing a regulatory oligonucleotide with a melting temperature Tm2, comprising a sequence segment that is at least partially complementary to the regulatory sequence, amplifying the tagged target nucleic acid sequence in a PCR amplification reaction using the probe oligonucleotide as a primer, and detecting the amplification product; wherein Tm1 and Tm2 are higher than the annealing temperature associated with the polynucleotide amplification reaction. |
申请公布号 |
WO2012154876(A1) |
申请公布日期 |
2012.11.15 |
申请号 |
WO2012US37155 |
申请日期 |
2012.05.09 |
申请人 |
FLUIDIGM CORPORATION;LIVAK, KENNETH J.;MYERS, STACEY;WANG, XIAOHUI;WANG, JUN |
发明人 |
LIVAK, KENNETH J.;MYERS, STACEY;WANG, XIAOHUI;WANG, JUN |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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