| 摘要 |
PURPOSE: A silkworm egg for transformation for producing green fluorescence silk and a transformant from the same are provided to stably and cheaply produce useful materials. CONSTITUTION: A silkworm egg for transformation is prepared by acid treatment of an egg of dormant state at 25 Deg. C. for one hour. A method for preparing a silkworm transformant comprises: a step of preparing the silkworm egg; a step of cloning a cassette vector to a basic vector in which a red fluorescent gene is introduced at 3XP promoter downstream and preparing a delivery system for silkworm transformation; and a step of microinjection of the delivery system to the silkworm egg. [Reference numerals] (AA) Hatching rate(%); (BB, DD) Untreated; (CC, EE) Micro-injection
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