METHOD FOR DETERMINING A CONCENTRATION OF TARGET NUCLEIC ACID MOLECULES, NUCLEIC ACID PROBES FOR THE METHOD, AND METHOD FOR ANALYSING DATA OBTAINED BY THE METHOD

摘要

Provided is a nucleic acid probe for determining a concentration of a target nucleic acid, said probe being labeled with a fluorescent dye, wherein: said probe is labeled at an end portion thereof with said fluorescent dye; said probe has a base sequence designed such that, when said probe is hybridized with said target nucleic acid, at least one G (guanine) base exists in a base sequence of said target nucleic acid at a position 1 to 3 bases apart from an end base portion where said probe and said target nucleic acid are hybridized with each other; said fluorescent dye has a reduced fluorescence emission when said probe is a hybridized with said target nucleic acid; said probe has C (cytosine) as a 3'end base and is labeled on an OH group at the 3'-position of deoxyribose in the 3' end base with said fluorescent dye through a spacer containing from 4 to 7 methylene units; and the fluorescent dye is BODIPY FL, BODIPY 5-FAM, or BODIPY TR.