发明名称 |
Regioselective alkane hydroxylation with a mutant AlkB enzyme |
摘要 |
AlkB from Pseudomonas putida was engineered using in-vivo directed evolution to hydroxylate small chain alkanes. Mutant AlkB-BMO1 hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. Mutant AlkB-BMO2 similarly hydroxylates propane and butane at the terminal carbon at a rate greater than the wild-type to form 1-propanol and 1-butanol, respectively. These biocatalysts are highly active for small chain alkane substrates and their regioselectivity is retained in whole-cell biotransformations.
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申请公布号 |
US8309333(B1) |
申请公布日期 |
2012.11.13 |
申请号 |
US20090618960 |
申请日期 |
2009.11.16 |
申请人 |
KOCH DANIEL J.;ARNOLD FRANCES H.;THE UNITED STATES OF AMERICA, AS REPRESENTED BY DEPARTMENT OF ENERGY |
发明人 |
KOCH DANIEL J.;ARNOLD FRANCES H. |
分类号 |
C12N9/00;C07H21/04 |
主分类号 |
C12N9/00 |
代理机构 |
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地址 |
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