| 摘要 |
<p>A chromatography column 50 containing a bed of packed particles comprising fused core particles. The bed comprises a plurality of bed sections 51,53 and each bed section has an average particle diameter calculated from the particles in that section. There are at least two different average particle diameter bed sections, wherein the particles of each bed are separated from particles of an adjacent bed by a partition 78 that is liquid permeable to allow through a flow of mobile phase. Preferably each partition is a frit and the partition prevents particles from one section from mixing with particles of an adjacent section. In use, a liquid mobile phase containing a sample to be separated into components is flowed through the packed column. Advantageously a high column efficiency can be provided with lower pressure drop per unit length of the column.</p> |
