| 摘要 |
A chromatography column 2 containing a bed of packed particles, wherein the packed particles 22, 24, 26, 28, 30 comprise fused core particles and the particle diameters of the packed particles vary along the column. Preferably, the particles are arranged according to their average particle diameter, in order of increasing average particle diameter from the inlet end to the outlet end. The bed may comprise a plurality of bed sections and each bed section has an average particle diameter calculated from the particles in that section and there are at least two different average particle diameter bed sections, wherein the particles of each bed are separated from particles of an adjacent bed by a partition that is liquid permeable to allow through a flow of mobile phase. Advantageously a high column efficiency can be provided with lower pressure drop per unit length of the column. |
