| 摘要 |
PURPOSE: A method for preparing transformed E.coli for overexpression of fatty acid biosynthesis pathway is provided to enhance genetic stability of the transformed E.coli. CONSTITUTION: A method for preparing a co-transformed E.coli comprises: a step of inserting an aceE(pyruvate dehydrogenase, decarboxylase component E1) gene and accA(acetyl-CoA carboxylase, carboxytransferase) gene into an expression vector; and a step of electroporation of E.coli with the expression vector and transforming. A fatty acid biosynthesis comprises a step of culturing co-transformed E.coli and biosynthesizing fatty acid; and a step of collecting the synthesized fatty acid.
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