发明名称 |
Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same |
摘要 |
The subject invention provides a unidirectional site-specific integration system for integrating a nucleic acid into the genome of a target cell. The provided system includes a site-specific integrating expression cassette (INTEC) vector, consisting of (a) a polynucleotide of interest operably linked to a promoter, (b) a single recombination site, and (c) a hybrid recombination site. In using the subject systems for site-specific integration, the INTEC vector and integrase are introduced into the target cell and the cell is maintained under conditions sufficient to provide for site-specific integration of the nucleic acid into the target cell genome via a recombination event mediated by the site-specific recombinase. Also provided are kits that include the subject systems. The subjects systems, methods and kits find use in a variety of different applications, several representative ones of which are described in detail as well. |
申请公布号 |
US8304233(B2) |
申请公布日期 |
2012.11.06 |
申请号 |
US20050198885 |
申请日期 |
2005.08.04 |
申请人 |
CALOS MICHELE P.;POETIC GENETICS, LLC;THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY |
发明人 |
CALOS MICHELE P. |
分类号 |
C12N15/74;C12N15/00;C12N15/63 |
主分类号 |
C12N15/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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