MODIFIED POLYNUCLEOTIDES FOR REDUCING OFF-TARGET EFFECTS IN RNA INTERFERENCE
摘要
Methods and compositions for performing RNA interference with decreased off-target effects are provided. The methods and compositions permit effective and efficient applications of RNA interference to applications such as diagnostics and therapeutics through the use of modifications to the siRNA. Uniquely modified siRNAs have been developed that reduce off-target effects incurred in gene-silencing. The modifications comprise 2'-O-alkyl or mismatch modification(s) at specific positions on the sense and/or antisense strands.
申请公布号
CA2561868(C)
申请公布日期
2012.11.06
申请号
CA20052561868
申请日期
2005.03.31
申请人
DHARMACON, INC.;ROSETTA INPHARMATICS LLC
发明人
LEAKE, DEVIN;FEDOROV, YURIY;REYNOLDS, ANGELA;KHVOROVA, ANASTASIA;MARSHALL, WILLIAM;LINSLEY, PETER S.