The present invention relates to a method for the lysis of cells, especially bacterial cells and the isolation of nucleic acids. The sample is treated with lysis solution that comprises at least one liquid that is not miscible with water and heated. Afterwards the mixture is cooled down and water is added so that after cooling a two phase system is generated. The nucleic acids can be found in the aqueous phase.
申请公布号
WO2012146338(A1)
申请公布日期
2012.11.01
申请号
WO2012EP01363
申请日期
2012.03.28
申请人
MERCK PATENT GMBH;SLAGHUIS, JOERG;ROSSMANITH, PETER;FUCHS, SABINE;MESTER, PATRICK, JULIAN;WAGNER, MARTIN
发明人
SLAGHUIS, JOERG;ROSSMANITH, PETER;FUCHS, SABINE;MESTER, PATRICK, JULIAN;WAGNER, MARTIN