发明名称 |
PLURIPOTENT STEM CELL-DERIVED BROWN ADIPOCYTES, PLURIPOTENT STEM CELL-DERIVED CELL AGGREGATE, METHOD FOR PRODUCING SAME, AND CELL THERAPY AND MEDICAL THERAPY THEREFOR |
摘要 |
[Problem] To provide a method for producing brown adipocytes using pluripotent stem cells, and a method for producing a cell aggregate which is an intermediate product thereof, as well as a pluripotent stem cell-derived cell aggregate and pluripotent stem cell-derived brown adipocytes produced by the methods, and a cell therapy using the pluripotent stem cell-derived brown adipocytes. [Solution] This method for producing brown adipocytes using pluripotent stem cells is characterized in that a cell aggregate is formed from the pluripotent stem cells by a method comprising a step (A), and the brown adipocytes are obtained from the cell aggregate by a method comprising a step (B). (A) A step for non-adhesively culturing pluripotent stem cells in a serum-free environment in the presence of a hematopoietic cytokine, and preparing a cell aggregate. (B) A step for adhesively culturing the cell aggregate in the presence of a hematopoietic cytokine, to prepare brown adipocytes. |
申请公布号 |
WO2012147853(A1) |
申请公布日期 |
2012.11.01 |
申请号 |
WO2012JP61212 |
申请日期 |
2012.04.26 |
申请人 |
NATIONAL CENTER FOR GLOBAL HEALTH AND MEDICINE;DNAVEC CORPORATION;SAEKI KUMIKO;YUO AKIRA;NISHIO MIWAKO;KAWASAKI MASAKO;SAEKI KOICHI;HASEGAWA MAMORU |
发明人 |
SAEKI KUMIKO;YUO AKIRA;NISHIO MIWAKO;KAWASAKI MASAKO;SAEKI KOICHI;HASEGAWA MAMORU |
分类号 |
C12N5/077;A61K35/12;A61L27/00;A61P3/06;A61P3/10;A61P9/10;C12N5/10;C12N15/09 |
主分类号 |
C12N5/077 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|