METHOD FOR DETERMINING SKIN SENSITISATION POTENTIAL

摘要

The present invention provides an in vitro method for predicting the in vivo skin sensitization potential of chemical compounds using a combination of mammalian cell models with multiple endpoints analysis. The method comprises the steps of: 1- selection of the chemicals concentrations to be tested using cell viability assays 2-detection of expression levels of genes implicated in skin sensitization 3-detection of the activation status of intracellular signaling pathways 4- outputs from points 2 and 3 are assessed for a group of chemical compounds comprising known sensitizers and irritants (training compounds) and used to build a statistical classification model trained and optimized to classify unknown chemicals compounds (test compounds) as either sensitizers or non-sensitizers.