| 摘要 |
<P>PROBLEM TO BE SOLVED: To provide glycosylation engineering to generate proteins having an altered glycosylation pattern resulting in improved therapeutic values. <P>SOLUTION: A host cell is engineered for the expression of at least one nucleic acid encoding a glycoprotein-modifying glycosyltransferaseat an adjusted level, and is engineered such that they are capable of expressing a preferred range of a glycoprotein-modifying glycosyl transferase activity which increases complex N-linked oligosaccharides carrying bisecting GlcNAc. The present invention is directed to method for the generation of modified glycoforms of glycoproteins, for example antibodies, including whole antibody molecules, antibody fragments, or fusion proteins that include a region equivalent to the Fc region of an immunoglobulin, having an enhanced Fc-mediated cellular cytotoxicity, and to glycoproteins so generated. <P>COPYRIGHT: (C)2013,JPO&INPIT |
