发明名称 |
INSULIN-LIKE GROWTH FACTOR 1 RECEPTOR (IGF-1R) PROTEIN SRM/MRM ASSAY |
摘要 |
<p>The current disclosure provides for specific peptides from the Insulin-Like Growth Factor 1 Receptor (IGF-1R) protein and the derived ionization characteristics of those peptides that are advantageous for quantifying the IGF-1R directly in formalin fixed biological samples by the method of Selected Reaction Monitoring (SRM) mass spectrometry. Such fixed biological samples include: formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and formalin fixed and paraffin embedded tissue culture cells. IGF-1R protein is quantitated in biological samples by the method of SRM/MRM mass spectrometry by quantitating one or more of the peptides described herein. The peptides can be quantitated if they reside in a modified or an unmodified form. Examples of potentially modified forms of an IGF-1R peptides include those bearing phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence.</p> |
申请公布号 |
EP2517011(A1) |
申请公布日期 |
2012.10.31 |
申请号 |
EP20100843597 |
申请日期 |
2010.12.22 |
申请人 |
EXPRESSION PATHOLOGY, INC. |
发明人 |
KRIZMAN, DAVID, B.;HEMBROUGH, TODD;THYPARAMBIL, SHEENO |
分类号 |
G01N33/50;G01N33/68;G01N33/74 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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