| 摘要 |
A method of investigating the chemical affinity between one or more target substances and a set of one or more test substances comprises the step of placing a solution of the target substance in a non-flowing chamber 11 of a flow cell that is separated from a flow-through chamber 13 by a semi-permeable or porous membrane 15. In a second step, a carrier fluid that carries discrete batches of one or more test substances in solution is moved through the flow-through chamber. In a third step, the retardation and spreading of the test substances that results from their chemical interactions with the target substance is detected. The target substance may be a macromolecule, protein, peptide, nucleic acid, virus, carbohydrate, lipid, or polysaccharide. Preferably, the time taken for one or more test substances to reach a detector is measured, a longer time indicating a stronger interaction between the target and test substances. Test substances may be passed through the flow cell one by one or in combination. |
