NOVEL KEUNJORONG MOSAIC VIRUS IN CYNANCHUM SP., PRIMER FOR DETECTION OF THE VIRUS AND METHOD FOR DETECTING THE VIRUS USING THEREOF
摘要
<p>PURPOSE: A novel cynanchum wilfordii hemsley mosaic virus discovered from a cynanchum plant, a primer for detecting the same and a detecting method of the cynanchum wilfordii hemsley mosaic virus using the same are provided to enhance the detection efficiency of the novel virus by using a primer pair. CONSTITUTION: A cynanchum wilfordii hemsley mosaic virus gene includes base sequence describe in the sequence number 1(SEQ ID NO:1). A primer pair for detecting cynanchum wilfordii hemsley mosaic virus comprises a forward primer selected from primers described in the sequence number 2-4 and a reverse primer selected from pimers described in the sequence number 5-7. The Cynanchum wilfordii Hemsley mosaic virus includes the base sequence described in the sequence number 1. A detecting method of the Cynanchum wilfordii Hemsley mosaic virus comprises the following steps: separating RNA from a sample; performing RT-PCR by using the primer pair as a mold; and detecting the virus from the sample by analyzing the PCR product. The Cynanchum wilfordii Hemsley mosaic virus detecting kit includes the primer pair.</p>
申请公布号
KR20120119466(A)
申请公布日期
2012.10.31
申请号
KR20110037409
申请日期
2011.04.21
申请人
REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
发明人
LEE, SU HEON;NAM, MOON;CHOI, HONG SOO;KIM, JEONG SOO;LEE, JUN SEONG;PARK, SEOK JIN;KIM, JEONG SEON;LEE, TAE SOON