发明名称 |
METHOD FOR MEASURING NEUROTRANSMITTERS IN VIVO |
摘要 |
<p>A method is provided for in vivo detection of a biochemical substance in an animal by culturing neurofluocytes that stably express a receptor of the biochemical substances by transfecting cells with cDNA of the receptor and a tag that will emit a detectable energy in the presence of the biochemical substance, implanting the neurofluocyte into the animal's brain; and detecting the energy emission of the tag. In a first embodiment, the biochemical substance is a neurotransmitter, the tag is a fluophore, and the step of detecting includes forming an opening in the animal's skull and optically detecting fluorescent emissions using a two-photon laser scanning microscope. Multiple biochemical substances can be simultaneously detected by culturing neurofluocytes that express different receptors and have different fluophor tags that produce fluorescent signals at distinguishable wavelengths.</p> |
申请公布号 |
EP2198294(A4) |
申请公布日期 |
2012.10.31 |
申请号 |
EP20080829655 |
申请日期 |
2008.09.04 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
NGUYEN, QUOC-THANG;KLEINFELD, DAVID;SCHROEDER, LEE F. |
分类号 |
G01N33/48;A61B5/00 |
主分类号 |
G01N33/48 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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