CULTURE METHOD FOR CAUSING DIFFERENTIATION OF PLURIPOTENT MAMMALIAN CELLS

摘要

Provided is a method that achieves control of embryoid body size and can induce differentiation in a state where the embryoid bodysize is controlled, by using a cell culture chamber having a plurality of microchambers formed therein. A culture method for causing differentiation of pluripotent mammalian cells uses a cell culturechamber (10) having a plurality of microchambers (11) formed on a culture surface. The cell culture chamber (10) has a culture surfaceformed of spaces in which the microchambers (11) have a spacestructure with a height of 10 gm to 500 pm and a bottom area of 100 pm2 to 1 mm2. The culture method for causing differentiation of pluripotent mammalian cells includes culturing pluripotent mammaliancells to obtain a cell population at least partially differentiated intoendoderm lineage cells, by using the cell culture chambers (10).