DETECTION METHOD FOR METALLOTHIONEIN USING QUARTZ CRYSTAL MICROBALANCE IMMUNOSENSOR

摘要

PURPOSE: A human anti-epithelial cell growth factor receptor Fab antibody and a detection method thereof are provided to detect metallothionine included in a sample up to the concentration of 0.1 nanogram/ml. CONSTITUTION: A detecting method of metallothionine included in a sample comprises the following steps: conflict reacting metallothionine fixed on the electrode surface of quartz crystal microbalance(QCM) chip with a sample for primary antibody; reacting the primary antibody combined with the fixed metallothionine with a secondary anti body in which horseradish peroxidase(HRP) is tagged; obtaining the frequency(F1) of vibration in a steady- state by adding a reactive buffer solution to the secondary antibody; obtaining the frequency(F2) of vibration in the steady- state by reacting 3,3'-diaminobenzidine(DAB) and H2O2 substrate solution with the secondary antibody in which the HRP is tagged; and measuring the vibration frequency variation(ΔF = F1-F2) from the frequency(F1) and the vibration frequency(F2).