METHOD FOR EVALUATION OF NATURAL AUTOANTIBODY LEVEL IN HUMAN BIOLOGICAL FLUIDS

摘要

FIELD: medicine. ^ SUBSTANCE: concentration of natural autoantibodies in human biological fluids is evaluated by enzyme immunoassay with the use of a solid phase of physical sorption coated with streptavidin. The solid phase of physical sorption is pre-processed with a biotin-modified antigen and a blocking agent for closing nonspecific binding sites. That is ensured by using proteins biotin-modified by standard technique; a conjugate-containing solution is presented by monoclonal or polyclonal enzyme-marked antibodies reacting with one or all isotopes of human immunoglobulins. A test sample is pre-dissolved in a buffer containins proteins used for closing nonspecific binding sites on the solid phase of physical sorption, thimerosal and Triton-X100. It is followed by treatment with ferric (III) chloride and thermal treatment. A reference solid phase of physical sorption whereon the biotin-modified antigen is not immobilised is applied for the test sample. The number of natural autoantibodies is evaluated by a calibration curve. ^ EFFECT: safety and effectiveness of evaluating the natural autoantibody level in human biological fluids. ^ 3 tbl, 1 dwg, 1 ex