CRYSTALLINE ANTI-HUMAN IL-12 ANTIBODIES

摘要

Disclosed is a batch crystallization method for crystallizing an anti-human IL-12 antibody, the method comprising the steps of: (a) providing an aqueous crystallization mixture having an aqueous solution of the antibody in admixture with at least one polyalkylene glycol having an average molecular weight in the range of about 400 to about 10,000 as a crystallization agent wherein the antibody concentration is in the range of about 0.5 to about 280 mg/ml; and (b) incubating the aqueous crystallization mixture at a pH of about pH 4 to about 6.5 and at a temperature of about 4o C to about 37o C until crystals of the antibody having a length of about 2-500 ìm are formed. Also disclosed is a crystal of an anti-human IL-12 antibody obtained by a batch crystallization method. Further disclosed is the use of the anti-human IL-12 antibody crystal in the manufacture of a medicament for the treatment of an IL-12 related disorder