OSCILLATING AMPLIFICATION REACTION FOR NUCLEIC ACIDS

摘要

One embodiment of the present invention provides for a method for amplifying a template of nucleic acid target sequence contained in a sample. The method includes contacting the sample with an amplification reaction mixture containing a primer complementary to the template of nucleic acid target sequence. A temperature of the reaction is oscillated between an upper temperature and a lower temperature wherein the change in temperature is no greater than about 20°C during a plurality of temperature cycles. The template of nucleic acid target sequence is amplified.