| 摘要 |
Methods are disclosed for detecting a nucleic acid lesion and obtaining sequence information comprising providing at least one nucleic acid adduct on the nucleic acid, wherein the nucleic acid adduct comprises a first current modulating compound coupled to a nucleotide of a first type, directing the nucleic acid into a channel (e.g. a nanopore) comprising an initial current in the absence of the nucleic acid, measuring the current in the channel when the nucleic acid is in the channel, detecting the current modulating compound in the channel, registering the identity and location of the current modulating compound on the nucleic acid and assigning the current modulating compound as a registration marker on the nucleic acid, thereby providing a detectable reference point in the sequence of the nucleic acid. Also provided is a method for identifying the number of repeat nucleotides in at least a portion of a nucleic acid strand. |
