| 摘要 |
The present invention provides a method and apparatus for measuring the membrane potential of red blood cells using electrophoretic analysis that analyzes red blood cells whose membrane potentials are reversed due to red blood cell agglutination. The method includes: dipping an electrophoresis cell into an electrophoresis tank that contains an electrophoresis solution such that a current can flow at the middle of the electrophoresis tank; introducing a specimen for measuring red blood cells to a zero point S (base point) at the center of the electrophoresis cell; capturing the image of the electrophoresis tank with time; performing RGB (red, green, and blue) analysis on image data of each of the captured images; measuring the moving coordinates of the brightness of each B component from the base point for each pixel, thereby analyzing the images; and measuring variation in the electrophoresis direction indicated by the accumulation result of the moving coordinates of the analysis data toward a cathode and an anode. The apparatus includes: an electrophoresis device A that includes an electrophoresis tank 1a having three tanks 101a, 10a, and 101a and an electrophoresis cell 3a dipped into the central tank 10a; an image capturing device 6a that captures the image of the electrophoresis cell 3a with time; and an analyzing device 7a that analyzes image data of the captured image. The electrophoresis solution 2a is contained in the three tanks such that it can flow through the three tanks, and a current can flow through the electrophoresis solution 2a. |
